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KMID : 0545120180280040638
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 4 p.638 ~ p.644
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Immobilization of Xylanase Using a Protein-Inorganic Hybrid System
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Kumar Ashok
Patel Sanjay K. S.
Mardan Bharat
Pagolu Raviteja
Lestari Rowina
Jeong Seong-Hoon
Kim Tae-Doo
Haw Jung-Rim
Kim Sang-Yong
Kim In-Won
Lee Jung-Kul
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Abstract
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In this study, the immobilization of xylanase using a protein-inorganic hybrid nanoflower system was assessed to improve the enzyme properties. The synthesis of hybrid xylanase nanoflowers was very effective at 4°C for 72 h, using 0.25 mg/ml protein, and efficient immobilization of xylanase was observed, with a maximum encapsulation yield and relative activity of 78.5% and 148%, respectively. Immobilized xylanase showed high residual activity at broad pH and temperature ranges. Using birchwood xylan as a substrate, the Vmax and Km values of xylanase nanoflowers were 1.60 mg/ml and 455 μmol/min/mg protein, compared with 1.42 mg/ml and 300 μmol/min/mg protein, respectively, for the free enzyme. After 5 and 10 cycles of reuse, the xylanase nanoflowers retained 87.5% and 75.8% residual activity, respectively. These results demonstrate that xylanase immobilization using a proteininorganic hybrid nanoflower system is an effective approach for its potential biotechnological applications.
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KEYWORD
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Immobilization, encapsulation, protein-inorganic hybrid, reusability, Thermomyces lanuginosus, xylanase
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